cav2 flp Search Results


cav2-flex (loxp)-flp virus  (BioCampus Cologne)
90
BioCampus Cologne cav2-flex (loxp)-flp virus
Visualization of GABAergic neurons in the POA, which make monosynaptic input to HDC and orexin neurons. A, Schematic representation of the procedure. We injected <t>AAV10-FLEX-TVA-mCherry</t> and AAV10-FLEX-RG stereotaxically into the TMN of Gad67-GFPΔNeo;Hdc-Cre or the LHA of Gad67-GFPΔNeo;Orexin-Cre mice. After 14 d, SADΔG-mCherry(EnvA) was injected into the same areas and the brains were analyzed 4–7 d after injection. B, Representative images of distribution of labeled cells in POA of Gad67-GFPΔNeo;Hdc-Cre mice. C, Representative image of distribution of labeled cells in POA of Gad67-GFPΔNeo;Orexin-Cre mice. Green, GFP. Red, SADΔG-mCherry. D, GABAergic input neurons to HDC neurons in the VLPO. Right, High-power view of rectangular region in left panel. E, GABAergic input neurons to orexin neurons in VLPO. Right, High-power view of rectangular region in the panel. F, Percentages of double-positive GABAergic neurons among input neurons to HDC neurons or orexin neurons in designated POA areas. G, Analysis of axonal arborization pattern of GABAVLPO→LHA neurons. <t>CAV2-FLEX</t> <t>(loxP)Flp</t> was injected into the LHA in vGAT-ires-Cre mice. AAV10-CAG-FLEX (Frt)-TVA-ChR2-eYFP was injected into the POA to express ChR2-eYFP in GABAPOA→LHA cells. H, YFP+ neurons observed in the POA (left). YFP+ fibers make close appositions to orexin and HDC neurons (middle and right). I, Specimen responses of GABAVLPO→HDC neurons (top traces) and GABAVLPO→orexin neurons (bottom traces) to 30 s bath application of 100 μm NA or 100 μm 5-HT. J, Summary of changes in membrane potential of GABAVLPO→HDC neurons and GABAVLPO→orexin neurons induced by drugs.
Cav2 Flex (Loxp) Flp Virus, supplied by BioCampus Cologne, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/cav2+flp/pmc06596100-148-0-6?v=BioCampus+Cologne
Average 90 stars, based on 1 article reviews
cav2-flex (loxp)-flp virus - by Bioz Stars, 2026-08
90/100 stars
  Buy from Supplier
cav2 flp  (Addgene inc)
93
Addgene inc cav2 flp
Visualization of GABAergic neurons in the POA, which make monosynaptic input to HDC and orexin neurons. A, Schematic representation of the procedure. We injected <t>AAV10-FLEX-TVA-mCherry</t> and AAV10-FLEX-RG stereotaxically into the TMN of Gad67-GFPΔNeo;Hdc-Cre or the LHA of Gad67-GFPΔNeo;Orexin-Cre mice. After 14 d, SADΔG-mCherry(EnvA) was injected into the same areas and the brains were analyzed 4–7 d after injection. B, Representative images of distribution of labeled cells in POA of Gad67-GFPΔNeo;Hdc-Cre mice. C, Representative image of distribution of labeled cells in POA of Gad67-GFPΔNeo;Orexin-Cre mice. Green, GFP. Red, SADΔG-mCherry. D, GABAergic input neurons to HDC neurons in the VLPO. Right, High-power view of rectangular region in left panel. E, GABAergic input neurons to orexin neurons in VLPO. Right, High-power view of rectangular region in the panel. F, Percentages of double-positive GABAergic neurons among input neurons to HDC neurons or orexin neurons in designated POA areas. G, Analysis of axonal arborization pattern of GABAVLPO→LHA neurons. <t>CAV2-FLEX</t> <t>(loxP)Flp</t> was injected into the LHA in vGAT-ires-Cre mice. AAV10-CAG-FLEX (Frt)-TVA-ChR2-eYFP was injected into the POA to express ChR2-eYFP in GABAPOA→LHA cells. H, YFP+ neurons observed in the POA (left). YFP+ fibers make close appositions to orexin and HDC neurons (middle and right). I, Specimen responses of GABAVLPO→HDC neurons (top traces) and GABAVLPO→orexin neurons (bottom traces) to 30 s bath application of 100 μm NA or 100 μm 5-HT. J, Summary of changes in membrane potential of GABAVLPO→HDC neurons and GABAVLPO→orexin neurons induced by drugs.
Cav2 Flp, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/cav2+flp/pm33861951-506-37-53?v=Addgene+inc
Average 93 stars, based on 1 article reviews
cav2 flp - by Bioz Stars, 2026-08
93/100 stars
  Buy from Supplier

Image Search Results


Visualization of GABAergic neurons in the POA, which make monosynaptic input to HDC and orexin neurons. A, Schematic representation of the procedure. We injected AAV10-FLEX-TVA-mCherry and AAV10-FLEX-RG stereotaxically into the TMN of Gad67-GFPΔNeo;Hdc-Cre or the LHA of Gad67-GFPΔNeo;Orexin-Cre mice. After 14 d, SADΔG-mCherry(EnvA) was injected into the same areas and the brains were analyzed 4–7 d after injection. B, Representative images of distribution of labeled cells in POA of Gad67-GFPΔNeo;Hdc-Cre mice. C, Representative image of distribution of labeled cells in POA of Gad67-GFPΔNeo;Orexin-Cre mice. Green, GFP. Red, SADΔG-mCherry. D, GABAergic input neurons to HDC neurons in the VLPO. Right, High-power view of rectangular region in left panel. E, GABAergic input neurons to orexin neurons in VLPO. Right, High-power view of rectangular region in the panel. F, Percentages of double-positive GABAergic neurons among input neurons to HDC neurons or orexin neurons in designated POA areas. G, Analysis of axonal arborization pattern of GABAVLPO→LHA neurons. CAV2-FLEX (loxP)Flp was injected into the LHA in vGAT-ires-Cre mice. AAV10-CAG-FLEX (Frt)-TVA-ChR2-eYFP was injected into the POA to express ChR2-eYFP in GABAPOA→LHA cells. H, YFP+ neurons observed in the POA (left). YFP+ fibers make close appositions to orexin and HDC neurons (middle and right). I, Specimen responses of GABAVLPO→HDC neurons (top traces) and GABAVLPO→orexin neurons (bottom traces) to 30 s bath application of 100 μm NA or 100 μm 5-HT. J, Summary of changes in membrane potential of GABAVLPO→HDC neurons and GABAVLPO→orexin neurons induced by drugs.

Journal: The Journal of Neuroscience

Article Title: Monoamines Inhibit GABAergic Neurons in Ventrolateral Preoptic Area That Make Direct Synaptic Connections to Hypothalamic Arousal Neurons

doi: 10.1523/JNEUROSCI.2835-17.2018

Figure Lengend Snippet: Visualization of GABAergic neurons in the POA, which make monosynaptic input to HDC and orexin neurons. A, Schematic representation of the procedure. We injected AAV10-FLEX-TVA-mCherry and AAV10-FLEX-RG stereotaxically into the TMN of Gad67-GFPΔNeo;Hdc-Cre or the LHA of Gad67-GFPΔNeo;Orexin-Cre mice. After 14 d, SADΔG-mCherry(EnvA) was injected into the same areas and the brains were analyzed 4–7 d after injection. B, Representative images of distribution of labeled cells in POA of Gad67-GFPΔNeo;Hdc-Cre mice. C, Representative image of distribution of labeled cells in POA of Gad67-GFPΔNeo;Orexin-Cre mice. Green, GFP. Red, SADΔG-mCherry. D, GABAergic input neurons to HDC neurons in the VLPO. Right, High-power view of rectangular region in left panel. E, GABAergic input neurons to orexin neurons in VLPO. Right, High-power view of rectangular region in the panel. F, Percentages of double-positive GABAergic neurons among input neurons to HDC neurons or orexin neurons in designated POA areas. G, Analysis of axonal arborization pattern of GABAVLPO→LHA neurons. CAV2-FLEX (loxP)Flp was injected into the LHA in vGAT-ires-Cre mice. AAV10-CAG-FLEX (Frt)-TVA-ChR2-eYFP was injected into the POA to express ChR2-eYFP in GABAPOA→LHA cells. H, YFP+ neurons observed in the POA (left). YFP+ fibers make close appositions to orexin and HDC neurons (middle and right). I, Specimen responses of GABAVLPO→HDC neurons (top traces) and GABAVLPO→orexin neurons (bottom traces) to 30 s bath application of 100 μm NA or 100 μm 5-HT. J, Summary of changes in membrane potential of GABAVLPO→HDC neurons and GABAVLPO→orexin neurons induced by drugs.

Article Snippet: CAV2-FLEX (loxP)-Flp virus was obtained from BioCampus Montpellier.

Techniques: Injection, Labeling